Gary Ruvkun
Professor of Genetics, Harvard Medical School
Department of Molecular Biology, Massachusetts General Hospital
Simches Research Building
185 Cambridge Street, Boston, MA 02114
phone: 617-726-5959
email: ruvkun@molbio.mgh.harvard.edu
Ruvkun Lab home page:
https://www.ruvkun.hms.harvard.edu/
Major Research Interests
microRNA and RNA interference mechanisms, bacterial and animal genetic analysis of microbiome interactions, neuroendocrine control of detoxification, immunity, and aging, life on other planets.
Academic trajectory
1985 - present Asst, Assoc., Professor of Genetics, Harvard Medical School
1982 - 1985 Junior Fellow, Society of Fellows, Harvard University
1982 Ph.D. Harvard University (Biophysics)
1973 A.B. University of California at Berkeley (Biophysics)
Honors
2004 Rosenstiel Award, Brandeis University (w/ Victor Ambros, Andy Fire, Craig Mello)
2008 National Academy of Sciences
2008 Benjamin Franklin Medal, Franklin Institute (w/ Victor Ambros and David Baulcombe)
2008 Albert Lasker Award for Basic Medical Research (w/Victor Ambros and David Baulcombe)
2008 Canada Gairdner International Award (w/ Victor Ambros)
2008 Warren Triennial Prize, Massachusetts General Hospital (w/ Victor Ambros)
2009 Louisa Gross Horwitz Prize, Columbia University (w/ Victor Ambros)
2009 American Academy of Arts and Sciences
2009 Shaul and Meira Massry Prize (w/ Victor Ambros)
2009 National Academy of Medicine
2010 Académie Royale des Sciences, des Lettres et des Beaux-Arts de Belgique
2011 Dan David Prize (w/ Cynthia Kenyon)
2012 Paul Janssen Award for Biomedical Research (w/ Victor Ambros)
2013 Ipsen Foundation Longevity Prize
2013 Irving Wright Award, American Foundation for Aging Research
2014 Wolf Prize in Medicine (w/ Victor Ambros)
2014 Gruber Genetics Prize (w/ Victor Ambros and David Baulcombe)
2015 Breakthrough Prize in Life Sciences (w/ Victor Ambros)
2016 March of Dimes Prize in Developmental Biology (w/ Victor Ambros)
2019 American Philosophical Society
Bibliography
Publications in reverse chronological order:
190. Meisel, JD, P Wiesenthal, V. Mootha, and G Ruvkun. 2024. Activation of a neuron-specific mitochondrial complex I subunit by the RNA methyltransferase CMTR-1. Current Biology, resubmitted 2024.
189. Kniazeva, M and Gary Ruvkun 2024 Activation of toxin defense of the ribosome by frameshift fusion of overlapping reading frames, submitted to Current Biology 2024.
188. Patange, O, P Breen, G Ruvkun 2023. Perturbed sulfur homeostasis allows C. elegans to escape growth retardation on Actinobacteria from its natural microbiome. 2023 bioRxiv 2023.11.12.566774; doi: https://doi.org/10.1101/2023.11.12.566774.
187. Yang, X, Meng, F, Liu, D, Gong, X, G Ruvkun and W Wei 2024 ATR surveils mitochondrial fission upon improper chromosome segregation in C. elegans, iBio, submitted 2024.
186. Yanagi, K, Jochimm B, Kunjo, SO, Breen, P, G Ruvkun and N Lehrbach. 2024 Nucleotide metabolism mutations bypass proteasome defects in png-1/NGLY1 deficient Caenorhabditis elegans. bioRxiv post 2023. PloS Biology, submitted.
185. Meisel JD, Miranda M, Skinner OS, Wiesenthal PP, Wellner SM, Jourdain AA, Ruvkun G, Mootha VK. Hypoxia and intra-complex genetic suppressors rescue complex I mutants by a shared mechanism. Cell. 2024 5:S0092-8674(23)01342-9. doi: 10.1016/j.cell.2023.12.010. PMID: 38215760
184. Warnhoff K, Snoozy J, Bhattacharya, S,Breen PC, Ruvkun G. 2024 Hypoxia-inducible factor promotes cysteine homeostasis in Caenorhabditis elegans (2024) eLife 12:RP89173. https://doi.org/10.7554/eLife.89173.3 bioRxiv. PMID: 37205365 bioRxiv. 2023 Nov 1:2023.05.04.538701. doi: 10.1101/2023.05.04.538701.
183. Mao K, Breen P, Ruvkun G. The Caenorhabditis elegans ARIP-4 DNA helicase couples mitochondrial surveillance to immune, detoxification, and antiviral pathways. Proc Natl Acad Sci 2022 Dec 6;119(49):e2215966119. doi: 10.1073/pnas.2215966119. 22 Nov 29. PMID: 36445965
182. Ji F, Bonilla G, Krykbaev R, Ruvkun G, Tabach Y, Sadreyev RI. DEPCOD: a tool to detect and visualize co-evolution of protein domains. 2022. Nucleic Acids Res. 2022:gkac349. doi: 10.1093/nar/gkac349. PMID: 35536332
181. Craven E, M Winters, AL Smith, E Lalime, R Mancinelli, B Shirey, W Schubert, A Schuerger, M Burgin, EP Seto, M Hendry, A Mehta, JN Benardini, G Ruvkun 2021. Biological safety in the context of backward planetary protection and Mars sample return: conclusions from the sterilization working group 2021. International Journal of Astrobiology 20: 1-28.
180. Ruvkun, G, F Ji, and RI Sadreyev. 2021. Phylogenetic evidence for asparagine to aspartic acid protein editing of N-glycosylated SARS-CoV-2 viral proteins by NGLY1 deglycosylation/deamidation suggests an unusual vaccination strategy. bioRxiv posting September 12, 2021. doi: https://doi.org/10.1101/2021.09.11.459891
179. Bryan NC, Lebreton F, Gilmore M, Ruvkun G, Zuber MT, Carr CE. 2021 Genomic and Functional Characterization of Enterococcus faecalis Isolates Recovered From the International Space Station and Their Potential for Pathogenicity. Front Microbiol. 2021 Jan 11;11:515319. doi: 10.3389/fmicb.2020.515319. PMID: 33505359
178. Warnhoff, K, TW. Hercher, RR Mendel, G Ruvkun. 2021. Protein-bound molybdenum cofactor is bioavailable and rescues molybdenum cofactor-deficient C. elegans, Genes Dev. 2021 Jan 14. doi: 10.1101/gad.345579.120. PMID: 33446569 bioRxiv 2020.10.08.332338; doi: https://doi.org/10.1101/2020.10.08.332338
177. Charlesworth. AG *, U Seroussi* , NJ Lehrbach*, MS Renaud , AE Sundby, RI Molnar, RX Lao , AR Willis, JR Woock, MJ Aber, AJ Diao, AW Reinke, GRuvkun, JM Claycomb. 2021. Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility. Nucleic Acids Res 2021 Sep 7;49(15):8836-8865. doi: 10.1093/nar/gkab619. PMID: 34329465 PMCID: PMC8421154 DOI: 10.1093/nar/gkab619.
176. Mao K, P Breen, G Ruvkun 2020. Mitochondrial dysfunction induces RNA interference in C. elegans through a pathway homologous to the mammalian RIG-I antiviral response. PLoS Biol. 2020 Dec 2;18(12):e3000996. doi: 10.1371/journal.pbio.3000996. PMID: 33264285
175. Govindan, JA, E Jayamani, V Lelyveld, J Szostak, and G Ruvkun. 2020. Bacterial carotenoids suppress Caenorhabditis elegans surveillance and defense of translational dysfunction. bioRxiv 2020.01.08.898668; doi: https://doi.org/10.1101/2020.01.08.898668
174. Carr, CE, NC Bryant, KN Saboda, SA Bhattaru, G Ruvkun MT Zuber 2020. Nanopore sequencing at Mars, Europa, and microgravity conditions NPJ Microgravity 6:24. doi: 10.1038/s41526-020-00113-9. PMID: 32964110
173. Wei W and Gary Ruvkun 2020. Lysosomal activity regulates Caenorhabditis elegans mitochondrial dynamics through vitamin B12 metabolism bioRxiv 2020.04.20.049502; doi: https://doi.org/10.1101/2020.04.20.049502. Proc Natl Acad Sci USA. 2020 117(33):19970-19981. doi: 10.1073/pnas.2008021117. PMID: 32737159
172. Fischer, SEJ and G Ruvkun. 2020. Caenorhabditis elegans ADAR editing and the ERI-6/7/MOV10 RNAi pathway silence endogenous viral elements and LTR retrotransposons. Proc Natl Acad Sci USA 117(11):5987-5996. doi: 10.1073/pnas.1919028117. 20 Mar 2. PMID: 32123111
171. Govindan, JA, E Jayamani, and G Ruvkun. 2019. ROS-based lethality of C. elegans mitochondrial electron transport mutants grown on E. coli siderophore iron release mutants Proc Natl Acad Sci USA. 116(43):21651-21658. doi: 10.1073/pnas.1912628116. PMID: 31591219
170. Armakola M and G Ruvkun 2019. Regulation of Caenorhabditis elegans neuronal polarity by heterochronic genes. Proc Natl Acad Sci USA. pii: 201820928. doi: 10.1073/pnas.1820928116. PMID:31164416
169. Mojarro A, J Hachey, R Bailey, M Brown, R Doebler, G Ruvkun, MT Zuber, and CE Carr. 2019. Nucleic acid extraction and sequencing from low-biomass synthetic Mars analog soils for in situ life detection. Astrobiology. doi: 10.1089/ast.2018.1929. PMID:31204862
168. Ast T, Meisel JD, Patra S, Wang H, Grange RMH, Kim SH, Calvo SE, Orefice LL, Nagashima F, Ichinose F, Zapol WM, Ruvkun G, Barondeau DP, Mootha VK. 2019. Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis. Cell 177:1507-1521.e16. doi: 10.1016/j.cell.2019.03.045. 19 Apr 25. PMID: 31031004
167. Lehrbach NJ, PC Breen, and G Ruvkun. 2019. Protein sequence editing of SKN-1A/Nrf1 by peptide:N-glycanase controls proteasome gene expression. Cell 177:737-750.e15. doi: 10.1016/j.cell.2019.03.035. PMID: 31002798
166. Lehrbach NJ and G Ruvkun. 2019. ER-associated SKN-1A/Nrf1 mediates a cytoplasmic unfolded protein response and promotes longevity. Elife. 11;8. pii: e44425. doi: 10.7554/eLife.44425. PMID: 30973820
165. Warnhoff K and G Ruvkun. 2019. Molybdenum cofactor transfer from bacteria to nematode mediates sulfite detoxification. Nat Chem Biol. 15(5):480-488. doi: 10.1038/s41589-019-0249-y. 19 Mar 25. PMID: 30911177
164. Mao K, F Ji, PC Breen, A Sewell, M Han, R Sadreyev, and G Ruvkun. 2019. Mitochondrial dysfunction in C. elegans activates mitochondrial relocalization and nuclear hormone receptor-dependent detoxification genes. Cell Metab. 29(5):1182-1191.e4. doi: 10.1016/j.cmet.2019.01.022. PMID: 30799287
163. Kniazeva M and G Ruvkun. 2019. Rhizobium induces DNA damage in Caenorhabditis elegans intestinal cells. Proceedings of the National Academy of Sciences, Proc Natl Acad Sci USA. 116(9):3784-3792. doi: 10.1073/pnas.1815656116. PMID: 30808764
162. Lin XX, Sen I, Janssens GE, Zhou X, Fonslow BR, Edgar D, Stroustrup N, Swoboda P, Yates JR 3rd, Ruvkun G, Riedel CG. 2018. DAF-16/FOXO and HLH-30/TFEB function as combinatorial transcription factors to promote stress resistance and longevity. Nat Commun. 9(1):4400. doi: 10.1038/s41467-018-06624-0. PMID: 30353013
161. Tillman EJ, Richardson CE, Cattie DJ, Reddy KC, Lehrbach NJ, Droste R, Ruvkun G, Kim DH. 2018. Endoplasmic Reticulum Homeostasis Is Modulated by the Forkhead Transcription Factor FKH-9 During Infection. PMID:30287474
160. Pontefract A, Hachey J, Zuber MT, Ruvkun G, Carr CE 2018. Sequencing nothing: Exploring failure modes of nanopore sensing and implications for life detection. Life Sci Space Res (Amst). 18:80-86. doi: 10.1016/j.lssr.2018.05.004. PMID: 30100151
159. Carr CE, NC Bryan, KN Saboda, SA Bhattaru, G Ruvkun, MT Zuber. 2018. Acceleration profiles and processing methods for parabolic flight NPJ Microgravity. 4:14. doi: 10.1038/s41526-018-0050-3. eCollection 2018. PMID: 30109261
158. Alexander JK, JR Casani, L Chiao, DP Fidler, J Gabrynowicz, GS Hubbard, EH Levy, N Noonan, K Olden, F Raulin, G Ruvkun, MP Saunders, BA Simmons, PD Stabekis, A Steele, DH Smith. 2018. Review and Assessment of Planetary Protection Policy Development Processes. Committee on the Review of Planetary Protection Policy Development Processes, Space Studies Board. https://doi.org/10.17226/25172.
157. Newman MA, Ji F, Fischer SEJ, Anselmo A, Sadreyev RI, G Ruvkun. 2018. The surveillance of pre-mRNA splicing is an early step in C. elegans RNAi of endogenous genes. Genes Dev. 2018 May 1;32(9-10):670-681. doi: 10.1101/gad.311514.118. PMID: 29739806
156. Mojarro A, J Hachey , G Ruvkun, MT Zuber, & CE Carr. 2018. CarrierSeq: a sequence analysis workflow for low-input nanopore sequencing. BMC Bioinformatics. 19:108. doi: 10.1186/s12859-018-2124-3. PMID:29587645
155. Pontefract A, TF Zhu, VK Walker, H Hepburn, C Lui, MT Zuber, G Ruvkun, and CE Carr. 2017. Microbial Diversity in a Hypersaline Sulfate Lake: A Terrestrial Analog of Ancient Mars. Frontiers in Microbiology, section Extreme Microbiology, Front Microbiol. 2017 Sep 26;8:1819. doi: 10.3389/fmicb.2017.01819. PMID:29018418
154. Mojarro A, Ruvkun G, Zuber MT, Carr CE. 2017 Nucleic Acid Extraction from Synthetic Mars Analog Soils for in situ Life Detection. Astrobiology. 2017 Jul 13. doi: 10.1089/ast.2016.1535. PMID:28704064
153. Lehrbach NJ and G Ruvkun. 2016. Proteasome dysfunction triggers activation of SKN-1A/Nrf1 by the aspartic protease DDI-1. Elife. 2016 Aug 16;5. pii: e17721. doi: 10.7554/eLife.17721. PMID:27528192
152. Dowen RH, PC Breen, T Tullius, AL Conery, and G Ruvkun. 2016. A microRNA program in the C. elegans hypodermis couples to intestinal mTORC2/PQM-1 signaling to modulate fat transport. Genes Dev. 2016 Jul 1;30(13):1515-28. doi: 10.1101/gad.283895.116. PMID:27401555
151. Samuel BS, H Rowedder, C Braendle, MA Félix, G Ruvkun 2016. Caenorhabditis elegans responses to bacteria from its natural habitats. Proc Natl Acad Sci USA., 2016.. pii: 201607183. PMID:27317746
150. Rettberg P, Anesio AM, Baker VR, Baross JA, Cady SL, Detsis E, Foreman CM, Hauber E, Ori GG, Pearce DA, Renno NO, Ruvkun G, Sattler B, Saunders MP, Smith DH, Wagner D, Westall F 2016. Planetary Protection and Mars Special Regions-A Suggestion for Updating the Definition. Astrobiology. 16(2):119-25. doi: 10.1089/ast.2016.1472. PMID: 26848950.
149. Govindan JA, Jayamani E, Zhang X, Mylonakis E, and G Ruvkun 2015. Dialogue between E. coli free radical pathways and the mitochondria of C. elegans. Proc Natl Acad Sci USA. pii: 201517448. PMID: 26392561
148. Govindan JA, E Jayamani, X Zhang, P Breen, J Larkins-Ford, E Mylonakis and G Ruvkun. 2015. Lipid signalling couples translational surveillance to systemic detoxification in Caenorhabditis elegans. Nat Cell Biol. 2015 Oct;17(10):1294-303. doi: 10.1038/ncb3229. PMID: 26322678. Author Correction: Nat Cell Biol. 2022 doi: 10.1038/s41556-022-00868-1. PMID: 35411089
147. Phillips CM, Brown KC, Montgomery BE, Ruvkun G, Montgomery TA. 2015. piRNAs and piRNA-dependent siRNAs protect conserved and essential C. elegans genes from misrouting into the RNAi pathway. Dev Cell. 2015 Aug 24;34(4):457-65. doi: 10.1016/j.devcel.2015.07.009. Aug 13.PMID:26279487
146. Sadreyev IR, Ji F, Cohen E, Ruvkun G, Tabach Y. 2015. PhyloGene server for identification and visualization of co-evolving proteins using normalized phylogenetic profiles. Nucleic Acids Res. Jul 1;43(W1):W154-9. doi: 10.1093/nar/gkv452. May 9. PMID: 25958392
145. Kirienko NV, FM Ausubel, and G Ruvkun 2015. Mitophagy confers resistance to siderophore-mediated killing by Pseudomonas aeruginosa. Proc Natl Acad Sci USA., 2015 Feb 10;112(6):1821-6. doi: 10.1073/pnas.1424954112. Jan 26. PMID:25624506
144. Wang MC, HD Oakley, CE Carr, JN Sowa, G Ruvkun 2014. Gene pathways that delay Caenorhabditis elegans reproductive senescence. PLoS Genet. 2014 Dec 4;10(12):e1004752. doi: 10.1371/journal.pgen.1004752. eCollection 2014 Dec. PMID: 25474471
143. Garcia SMDA, Y Tabach, G Lourenço, and G Ruvkun 2014. Identification of genes in toxicity pathways of trinucleotide-repeat RNA in C. elegans. Nature Structural and Molecular Biology, 2014 Aug;21(8):712-20. doi: 10.1038/nsmb.2858. Jul 20.PMID: 25038802.
142. Phillips CM, Rim Y, Roovers, E, Breen PC, Ohsumi, TK, van Wolfswinkel, JC, Ketting, RF, Ruvkun, G, TA Montgomery 2014. MUT-14 and SMUT-1 DEAD box RNA helicases have overlapping roles in germline RNAi and endogenous siRNA formation. Curr Biol. 2014 Apr 14;24(8):839-44. doi: 10.1016/j.cub.2014.02.060. Mar 27. PMCID: PMC4010136
141. Liu Y, BS Samuel, PC Breen and G Ruvkun 2014. Caenorhabditis elegans pathways that surveil and defend mitochondria. Nature. 2014 Apr 17;508(7496):406-10. doi: 10.1038/nature13204. Apr 2. PMCID: PMC4102179
140. Schwartz S, Agarwala SD, Mumbach MR, Jovanovic M, Mertins P, Shishkin A, Tabach Y, Mikkelsen TS, Satija R, Ruvkun G, Carr SA, Lander ES, Fink GR, Regev A. 2013. High-resolution mapping reveals a conserved, widespread, dynamic mRNA methylation program in yeast meiosis. Cell. 2013 Dec 5;155(6):1409-21. doi: 10.1016/j.cell.2013.10.047. Nov 21. PMCID: PMC3956118
139. Fischer SEJ, Q Pan, PC. Breen, Y Qi, Z Shi, C Zhang, TA Montgomery, and G Ruvkun 2013. Multiple small RNA pathways regulate the silencing of repeated and foreign genes in C. elegans. Genes and Development, 2013 Dec 15;27(24):2678-95. doi: 10.1101/gad.233254.113, PMCID: PMC3877757
138. Soukas AA, CE Carr, and G Ruvkun 2013. Genetic Regulation of C. elegans Lysosome Related Organelle Function. PloS Genetics, Oct;9(10):e1003908. doi: 10.1371/journal.pgen.1003908. Oct 24. PMCID: PMC3812091
137. Tabach Y, T Golan, A Hernández-Hernández AR Messer, T Fukuda, A Kouznetsova, JG Liu, I Lilienthal, C Levy, and G Ruvkun 2013. Human disease locus discovery and mapping to molecular pathways through phylogenetic profiling. Molecular Systems Biology, Oct 1;9:692. doi: 10.1038/msb.2013.50. PMCID: PMC3817400
136. Shi Z, G Hayes, and G Ruvkun 2013. Dual regulation of the lin-14 target mRNA by the lin-4 miRNA. PloS One, Sep 13;8(9):e75475. doi: 10.1371/journal.pone.0075475. PMCID: PMC3772890
135. Carr CE, Rowedder H, Lui CS, Zlatkovsky I, Papalias CW, Bolander J, Myers JW, Bustillo J, Rothberg JM, Zuber MT, and G Ruvkun 2013. Radiation Resistance of Sequencing Chips for in situ Life Detection. Astrobiology. 2013 Jun 4. PMID: 23734755
134. O’Rourke EA and G Ruvkun 2013. MXL-3 and HLH-30 transcriptionally link lipolysis and autophagy to nutrient availability. Nature Cell Biology, 2013 Jun;15(6):668-76. doi: 10.1038/ncb2741. Apr 21. PMCID: PMC3723461
133. Kirienko NV, DR Kirienko, J Larkins-Ford, C Wählby, G Ruvkun, FM Ausubel 2013 Pseudomonas aeruginosa disrupts Caenorhabditis elegans iron homeostasis, causing a hypoxic response and death. Cell Host and Microbe, 13(4):406-16. doi: 10.1016/j.chom.2013.03.003. PMCID: PMC3641844
132. Riedel CG, G Lima, NV. Kirienko, T Heimbucher, JA West, A Dillin, J Asara, and G Ruvkun 2013. DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. Nature Cell Biology, 2013 May;15(5):491-501. doi: 10.1038/ncb2720. Apr 21. PMCID: PMC3748955
131. O’Rourke EA, P Kuballa, R Xavier, and G Ruvkun 2013. w-6 Polyunsaturated fatty acids extend life span through the activation of autophagy. Genes and Development Feb 15;27(4):429-40. doi: 10.1101/gad.205294.112. Feb 7. PMCID: PMC3589559
130. Shi Z, T Montgomery, Y Qi and G Ruvkun 2013. High-throughput sequencing reveals extraordinary fluidity of miRNA, piRNA and siRNA pathways in nematodes. Genome Research Mar;23(3):497-508. doi: 10.1101/gr.149112.112. Jan 30. PMCID: PMC3589538
129. Tabach Y, A Billi, G Hayes, O Zuk, H Gabel, R Kamath, M Newman, K Yacoby, B Chapman, M Borowsky, J Kim, and G Ruvkun 2013. Identification of small RNA pathway genes using patterns of phylogenetic conservation and divergence. Nature Jan 31;493(7434):694-8. doi: 10.1038/nature11779. Dec 23. PMCID: PMC3762460
128. Carr CE, H Rowedder, C Vafadari, CS Lui, E Cascio, MT Zuber, and G Ruvkun 2013. Radiation resistance of biological reagents for in-situ life detection. Astrobiology Jan;13(1):68-78. doi: 10.1089/ast.2012.0869. PMID: 23330963
127. Tacutu R, Shore DE, Budovsky A, de Magalhães JP, Ruvkun G, Fraifeld VE, Curran SP. Prediction of C. elegans Longevity Genes by Human and Worm Longevity Networks 2012. PLoS One 2012;7(10):e48282. doi: 10.1371/journal.pone.0048282. Oct 29. PMCID: PMC3483217
126. Wählby C, Kamentsky L, Liu ZH, Riklin-Raviv T, Conery AL, O'Rourke EJ, Sokolnicki KL, Visvikis O, Ljosa V, Irazoqui JE, Golland P, Ruvkun G, Ausubel FM, Carpenter AE 2012. An image analysis toolbox for high-throughput C. elegans assays Nat Methods 9:714-6. doi: 10.1038/nmeth.1984. PMCID: PMC3433711
125. Shore DE, CE Carr and G Ruvkun 2012. Induction of cytoprotective pathways is central to the extension of lifespan conferred by multiple longevity pathways. PloS Genetics 8(7):e1002792. PMCID: PMC3400582
124. Phillips CM, PC Breen, TA Montgomery and G Ruvkun 2012. MUT-16 promotes formation of perinuclear mutator foci required for RNA silencing in the C. elegans germline. Genes and Development 26:1433-44. Jun 19. PMCID: PMC3403012
123. Zhang C, TA Montgomery, SE Fischer, SM Garcia, CG Riedel, N Fahlgren, CM Sullivan, JC Carrington and G Ruvkun 2012. The Caenorhabditis elegans RDE-10/RDE-11 complex regulates RNAi by promoting secondary siRNA amplification. Current Biology 22:881-90. Apr 26. PMCID: PMC3371361
121. Melo JA and G Ruvkun 2012. Inactivation of conserved C. elegans genes engages pathogen- and xenobiotic-associated defenses. Cell 149:452-66. PMCID: PMC3613046
120. Montgomery TA, Rim, E, Zhang, C, Dowen RH, Phillips CM, Fischer, SEJ and G. Ruvkun 2012. PIWI associated siRNAs and piRNAs specifically require the C. elegans HEN1 ortholog henn-1. PLoS Genetics, Apr;8(4):e1002616. Apr 19. PMCID: PMC3334881
119. Shi Z and G. Ruvkun 2012. The mevalonate pathway regulates microRNA activity in Caenorhabditis elegans. Proc Natl Acad Sci USA. Mar 20;109(12):4568-73. Mar 6. PMCID: PMC3311396
118. Wu X, Z Shi, M Cui, M Han and G. Ruvkun 2012. Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes. PLoS Genet. Mar;8(3):e1002542. Mar 8. PMCID: PMC3297578
117. Kimura KD, Riddle DL, and G. Ruvkun 2011 The C. elegans DAF-2 insulin-like receptor is abundantly expressed in the nervous system and regulated by nutritional status. Cold Spring Harb Symp Quant Biol. 76:113-20. Nov 28. PMID: 22123849
116. Russel S, AR Frand, and G Ruvkun 2011. Regulation of the C. elegans molt by pqn-47. Developmental Biology 360: 297-309. PMCID: PMC3618673
115. Fischer SEJ, TA Montgomery, C Zhang, N Fahlgren, A Hwang, CM Sullivan, JC Carrington and G Ruvkun 2011. The ERI-6/7 helicase acts at the first stage of an siRNA amplification pathway that targets recent gene duplications. PLoS Genet. Nov;7(11):e1002369. Nov 10. PMCID: PMC3213143
114. Hayes GD, C Riedel, and G Ruvkun 2011. The Caenorhabditis elegans SOMI-1 zinc finger protein and SWI/SNF promote regulation of development by the mir-84 microRNA. Genes Dev. Oct 1;25(19):2079-92. PMCID: PMC3197206
113. Wang MC, W Min, G Ruvkun, XS Xie 2011. RNAi screening for fat regulatory genes with SRS microscopy. Nature Methods, Feb;8(2):135-8. Jan 16. PMCID: PMC3061290
112. Zhang C, TA Montgomery, HW Gabel, SE Fischer, CM Phillips, N Fahlgren, C Sullivan, JC Carrington, and G Ruvkun 2011. mut-16 and other mutator-class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans. Proc. Natl. Acad. Sci., Jan 25;108(4):1201-8. Jan 18. PMCID: PMC3029761
111. Meli VS , Osuna B, Ruvkun G, Frand AR 2010. MLT-10 Defines a Family of DUF644 and Proline-rich Repeat Proteins Involved in the Molting Cycle of Caenorhabditis elegans. Mol Biol Cell. May 15;21(10):1648-61. PMCID: PMC2869372
110. Sebastiani P, Montano M, Puca A, Solovieff N, Kojima T, Wang MC, Melista E, Meltzer M, Fischer SE, Andersen S, Hartley SH, Sedgewick A, Arai Y, Bergman A, Barzilai N, Terry DF, Riva A, Anselmi CV, Malovini A, Kitamoto A, Sawabe M, Arai T, Gondo Y, Steinberg MH, Hirose N, Atzmon G, Ruvkun G, Baldwin CT, Perls TT 2009. RNA editing genes associated with extreme old age in humans and with lifespan in C. elegans. PLoS One. 2009 Dec 14;4(12):e8210. PMCID: PMC2788130
109. O'Rourke EJ, Soukas AA, Carr CE, G Ruvkun 2009. C. elegans major fats are stored in vesicles distinct from lysosome-related organelles. Cell Metab.10(5):430-5. PMCID: PMC2921818
108. Curran SP, X Wu, C Riedel, and G Ruvkun 2009. A soma-to-germline transformation in long-lived C. elegans mutants. Nature 459: 1079-84. PMCID: PMC2716045
107. Butcher RA, JR Ragains, G Ruvkun, J Clardy, HY Mak. 2009 Biosynthesis of the C. elegans dauer pheromone. Proceedings of the National Academy of Sciences 106(6):1875-9. PMCID: PMC2631283
106. Soukas AA, EA Kane, CE Carr, JA Melo, and G Ruvkun 2009. Rictor/TORC2 regulates fat metabolism, feeding, growth, and lifespan in Caenorhabditis elegans. Genes and Development 23: 496-511. PMCID: PMC2648650
105. Isenbarger TA, CE Carr, SS Johnson, M Finney, GM Church, W Gilbert, MT Zuber, and G Ruvkun 2008. The most conserved genome segments for life detection on Earth and other planets, Origin of Life and Evolution of Biospheres, 38(6):517-33. Oct 14. PMID: 18853276
104. Wang MC, E O’Rourke and G Ruvkun 2008. Fat metabolism links germline stem cells and longevity in C. elegans. Science 322: 957-60. PMCID: PMC2760269
103. Fischer SEJ, MD Butler, Q Pan and G Ruvkun 2008. Trans-splicing in C. elegans generates the negative RNAi regulator ERI-6/7. Nature 455: 491-6. PMCID: PMC2756026
102. Simon DJ, Madison JM, Conery AL, Thompson-Peer KL, Soskis M, Ruvkun G, Kaplan JM, Kim JK 2008. The microRNA miR-1 regulates a MEF-2-dependent retrograde signal at neuromuscular junctions. Cell 133: 903-15. PMCID: PMC2553566
101. Patel DS, Fang LL, Svy DK, Ruvkun G, Li W 2008. Genetic identification of HSD-1, a conserved steroidogenic enzyme that directs larval development in Caenorhabditis elegans. Development. 135: 2239-49. PMID: 18495818
100. Gabel HW and G. Ruvkun 2008. The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi. Nat Struct Mol Biol. 15: 531-3. PMCID: PMC2910399
99. Samuelson AV, RR Klimczak, D Thompson, CE Carr, and G Ruvkun 2008. Identification of C. elegans genes regulating longevity using enhanced RNAi-sensitive strains. Cold Spring Harb Symp Quant Biol. 2007;72:489-97. PMID: 18419309
98. Pierce ML, Weston MD, Fritzsch B, Gabel HW, Ruvkun G, Soukup GA 2008. MicroRNA-183 family conservation and ciliated neurosensory organ expression. Evol Dev. 10(1):106-13. PMCID: PMC2637451
97. Isenbarger TA, M Finney, C Ríos-Velázquez, J Handelsman, and G Ruvkun 2007. Miniprimer PCR, a new lens for viewing the microbial world. Appl Environ Microbiol. 2008 Feb;74(3):840-9. PMCID: PMC2227730
96. Parry DH, Xu, J and G Ruvkun 2007 A whole-genome RNAi screen for C. elegans miRNA pathway genes. Current Biology 17: 2013-22. PMCID: PMC2211719
95. Samuelson AV, Carr, CE, and G Ruvkun 2007. Gene activities that mediate increased lifespan of C. elegans insulin-like signaling mutants. Genes and Development 21:2976-94. PMCID: PMC2049198
94. Kim N, Dempsey C, Kuan CJ, Zoval J, O'Rourke E, Ruvkun G, Madou M, J Sze 2007. Gravity force transduced by the MEC-4/MEC-10 DEG/ENaC channel modulates DAF-16/FoxO activity in C. elegans. Genetics. 177:835-45. PMCID: PMC2034647
93. Curran SP and G Ruvkun 2007. Lifespan regulation by evolutionarily conserved genes essential for viability. PloS Genetics 3(4):e56. PMCID: PMC1847696
92. Hayes GD and G Ruvkun 2006. Misexpression of the C. elegans miRNA let‑7 is sufficient to drive developmental programs. Cold Spring Harbor Symp Quant Biol. 71: 21-7. Regulatory RNAs. PMID: 17381276
91. Ruvinsky I, U Ohler, CB Burge, and G Ruvkun 2006. Detection of broadly expressed neuronal genes in C. elegans. Dev Biol. 302: 617-26. PMID: 17046742
90. Hayes GD, AR Frand, and G. Ruvkun 2006. The mir-84 and let-7 paralogous microRNA genes of Caenorhabditis elegans direct the cessation of molting via the conserved nuclear hormone receptors NHR-23 and NHR-25. Development 133: 4631-4641. PMID: 17065234
89. Hu PJ, J Xu, and G Ruvkun 2006. Two membrane-associated tyrosine phosphatase homologs potentiate C. elegans AKT-1/PKB signaling. PLoS Genet. 2006 Jul;2(7):e99. PMCID: PMC1487177
88. Sandoval GM, JS Duerr, J Hodgkin, JB Rand and G Ruvkun 2006. A genetic interaction between the vesicular acetylcholine transporter VAChT/UNC-17 and synaptobrevin/SNB-1 in C. elegans. Nature Neuroscience, 9:599-601. PMID: 16604067
87. Mak HY, LS Nelson, M Basson, CD Johnson and G Ruvkun 2006. Polygenic control of C. elegans fat storage. Nature Genetics 38:363-8. PMID: 16462744
86. Duchaine TF, JA Wohlschlegel, S Kennedy, Y Bei, D Conte, K Pang, DR Brownell, S Harding, S Mitani, G Ruvkun, JR Yates, and CC Mello 2006. Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways. Cell 124:343-54. PMID: 16439208
85. Frand AR, S Russel, G Ruvkun 2005. Functional genomic analysis of C. elegans molting. PLoS Biol. 2005 Oct;3(10):e312. doi: 10.1371/journal.pbio.0030312. PMID: 16122351
84. Wang DS, S Kennedy, D Conte, J Kim, H Gabel, R Kamath, C Mello, and G Ruvkun 2005. Somatic misexpression of germline P granules and enhanced RNA interference in Retinoblastoma pathway mutants. Nature 436: 593-597. PMID: 16049496
83. Sieburth D, Q Ch ng, M Dybbs, M Tavazoie, S Kennedy, D Wang, D Dupuy, J Rual, D Hill, M Vidal, G Ruvkun, and J Kaplan 2005. Systematic analysis of genes required for synapse structure and function. Nature 436: 510-517. PMID: 16049479
82. Hamilton B , Y Dong, M Shindo, W Liu, G Ruvkun, and SS Lee 2005. A systematic RNAi screen for longevity genes in C. elegans. Genes and Development 19:1544-55. PMCID: PMC1172061
81. Efimenko E, Bubb K, Mak HY, Holzman T, Leroux MR, Ruvkun G, Thomas JH, Swoboda P 2005. Analysis of xbx genes in C. elegans. Development. 132:1923-34. PMID: 15790967
80. Kim JK, HW Gabel, RS Kamath, M Tewari, A Pasquinelli, S Kennedy, M Dybbs, N Bertin, M Vidal, JM Kaplan, and G Ruvkun 2005. Functional genomic analysis of RNA interference in C. elegans. Science 308: 1164-7. PMID: 15790806
79. Wang D and G Ruvkun 2004. The insulin pathway regulates RNAi in C. elegans. Cold Spring Harbor Symposium on Quantitative Biology, 69th edition, 69: 429-433.
78. Mansfield JH, Harfe BD, Nissen R, Obenauer J, Srineel J, Chaudhuri A, Farzan-Kashani R, Zuker M, Pasquinelli AE, Ruvkun G, Sharp PA, Tabin CJ, McManus MT 2004. MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nat Genet. 36:1079-83. PMID: 15361871
77. Mak HY and G Ruvkun 2004. Intercellular signaling of reproductive development by the C. elegans DAF-9 cytochrome P450. Development, 131:1777-86. PMID: 15084462
76. Tewari M, Hu PJ, Ahn JS, Ayivi-Guedehoussou N, Vidalain PO, Li S, Milstein S, Armstrong CM, Boxem M, Butler MD, Busiguina S, Rual JF, Ibarrola N, Chaklos ST, Bertin N, Vaglio P, Edgley ML, King KV, Albert PS, Vandenhaute J, Pandey A, Riddle DL, Ruvkun G, Vidal M 2004. Systematic interactome mapping and genetic perturbation analysis of a C. elegans TGF-beta signaling network. Molecular Cell 13:469-82. PMID: 14992718
75. Kennedy SK, D Wang, and G Ruvkun 2004. A conserved siRNA degrading RNase negatively regulates RNA interference in C. elegans. Nature 427: 645-9. PMID: 14961122
74. Kim J, Krichevsky, A, Grad, Y, Hayes, GD, Kosik, KS, Church, GM, and G Ruvkun 2004. Identification of many microRNAs that copurify with polyribosomes in mammalian neurons. Proc. Natl. Acad. Sci.101: 360-5. PMCID: PMC314190
73. Sze JY and G Ruvkun 2003. Activity of the Caenorhabditis elegans UNC-86 POU transcription factor modulates olfactory sensitivity, Proc. Natl. Acad. Sci., 100:9560-5. PMCID: PMC170957
72. Ruvinsky I and G Ruvkun 2003. Functional tests of enhancer conservation between distantly related species, Development, 130:5133-42. PMID: 12944426
71. Garsin DA. JM Villanueva, J Begun, DH Kim, CD Sifri, SB Calderwood, G Ruvkun, and FM Ausubel 2003. Long-Lived Caenorhabditis elegans daf-2 mutants are resistant to bacterial pathogens, Science, 300:1921. PMID: 12817143
70. Grad Y, Aach, J, Hayes, GD, Reinhart, B, Church, GM, Ruvkun, G, and J Kim. 2003. Computational and experimental identification of C. elegans microRNAs. Molecular Cell, 11:1253-63. PMID: 12769849
69. Lee SS, Kennedy,S, Tolonen, AC and G Ruvkun 2003. DAF-16 target genes that control C. elegans life-span and metabolism. Science. 300:644-7. PMID: 12690206
68. Aspöck G, G Ruvkun, and T Bürglin 2003. The Caenorhabditis elegans ems class homeobox gene ceh-2 is required for M3 pharynx motoneuron function. Development, 130:3369-3378. PMID: 12810585
67. Pasquinelli AE, McCoy, A Jimenez, E Salo, G Ruvkun, M Martindale, and J Baguñà 2003. Expression of the 22 nucleotide let-7 heterochronic RNA throughout the Metazoa: A role in life history evolution? Evolution and Development, 5:372-378. PMID: 12823453
66. Bashirullah A, Pasquinelli, AE Kiger, A, Perrimon, N, Ruvkun, G, and C S Thummel 2003. Coordinate regulation of small temporal RNAs at the onset of Drosophila metamorphosis. Developmental Biology, 259:1-8. PMID: 12812783
65. Li W and G Ruvkun 2003. daf-28 encodes a C. elegans insulin superfamily member that is regulated by environmental cues and acts in the DAF-2 signaling pathway. Genes and Development, 17: 844-58. PMCID: PMC196030
64. Ashrafi K, Chang, FY,Watts, JL, Fraser, AG, Kamath, RS, Ahringer, J and G Ruvkun 2003. Genome-wide RNAi analysis of C. elegans fat regulatory genes. Nature, 421:268-72. PMID: 12529643
63. Lee SS, Lee, RY, Fraser, AG, Kamath, RS, Ahringer, J and G Ruvkun 2002. A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity. Nature Genetics, 33:40-8. PMID: 12447374
62. Wolkow CA, Munoz MJ, Riddle DL, G Ruvkun 2002. Insulin receptor substrate and p55 orthologous adaptor proteins function in the Caenorhabditis elegans daf-2/insulin-like signaling pathway. J Biol Chem. 277:49591-7. PMID: 12393910
61. Ruvkun G, M Finney, W Gilbert, G Church. 2002. A Robotic-PCR Detector for DNA-based Life on Other Planets, in Signs of Life: A Report based on the April 2000 Workshop on Life Detection Techniques. Space Sciences Board, NASA.
60. Sze JY, Zhang, S, Li, J, and G Ruvkun 2002. The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1 tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic neurons. Development, 129:3901-11. PMID: 12135927
59. Lee RYN, Hench, J, and G Ruvkun 2001. Regulation of C. elegans DAF-16 and its human ortholog FKHRL1 by the daf-2 insulin-like signaling pathway. Curr Biol. 11: 1950-1957. PMID: 11747821
58. Grishok A, Pasquinelli, AE, Conte, D, Li, N, Parrish, S, Ha, I, Baillie, DL, Fire, A, Ruvkun, G, and Mello, CC 2001. Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control Caenorhabditis elegans developmental timing. Cell 106: 23-34. PMID: 11461699
57. Pierce SB, Costa, M, Wisotzkey, R , Devadhar, S, Homburger, SA, Buchman, AR, Ferguson, KC, Heller, J, Platt, DM , Liu, LX , Pasquinelli, AE, Doberstein, SK, G Ruvkun 2001. Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family. Genes and Development 15: 672-686. PMCID: PMC312654
56. Bürglin TR and G Ruvkun 2001. Regulation of ectodermal and excretory cell function by the C. elegans POU homeobox gene ceh-6 Development 128: 779-790. PMID: 11171402
55. Reinhart BJ and G Ruvkun 2001. Isoform-specific mutations in the Caenorhabitis elegans heterochronic gene lin-14 affect stage-specific patterning. Genetics 157: 199-209. PMCID: PMC1461488
54. Cahill CM, Tzivion, G, Nasrin, N, Ogg, S, Dore, J, Ruvkun, G, and M Alexander-Bridges 2001. Phosphatidylinositol 3-kinase signaling inhibits DAF-16 DNA binding and function via 14-3-3-dependent and 14-3-3-independent pathways. J. Biol. Chem. 276: 13402-10. PMID: 11124266
53. Wolkow CA, Kimura, KD, Lee, M and G Ruvkun 2000. Regulation of C. elegans life-span by insulinlike signaling in the nervous system. Science 290:147-50. PMID: 11021802
52. Pasquinelli A, Reinhart, B, Slack F , Maller, B, Kuroda, M, Martindale, M, Srinivasan, A, Fishman, M, Hayward D, Ball E, Degnan, B, Müller, P , Spring, J, Finnerty, J, Corbo, J, Levine, M, Leahy, P , Davidson, E, and G Ruvkun 2000. Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature 408: 86-89. PMID: 11081512
51. Nasrin N, Ogg, S, Cahill, C, Biggs, W, Nui, S, Dore, J, Calvo, D, Shi, Y, Ruvkun, G, and M Alexander-Bridges 2000. DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in HepG2 cells. Proc. Natl. Acad. Sci., 97: 10412-7. PMCID: PMC27038
50. Kagoshima H, Sommer, R, Reinhart, B , Ruvkun, G, Cassata, G, and TR Bürglin 2000. Graded expression of ceh-14 reporters in the hypodermis is induced by a gonadal signal. Development Genes and Evolution 210:564-569. PMID: 11180807
49. Slack FJ, Basson, M, Liu, Z, Ambros, V, Horvitz, HR, and G Ruvkun 2000. The lin-41 RBCC gene acts in the C. elegans heterochronic pathway between the let-7 regulatory RNA and the lin-29 transcription factor. Mol. Cell 5: 659-69. PMID: 10882102
48. Reinhart BJ, Slack, FA., Basson, M, Pasquinelli, AE, Bettinger, JC, Rougvie, AC, Horvitz, HR, and G Ruvkun 2000. The 21 nucleotide let-7 RNA regulates developmental timing in C. elegans. Nature, 403: 901 - 906. PMID: 10706289
47. Sze JY, Victor, M, Loer, C, Shi, Y, and G Ruvkun 2000. Food and metabolic signaling defects in a C. elegans serotonin-synthesis mutant, Nature 403: 560-4. PMID: 10676966
46. Tissenbaum HA Hawdon, J, Perregaux, M , Hotez, P, Guarente, L, and G Ruvkun 2000. A common muscarinic pathway for diapause recovery in the distantly related nematode species Caenorhabditis elegans and Ancylostoma caninum. Proc. Natl. Acad. Sci. 97: 460-465. PMCID: PMC26685
45. Sagasti A, Hobert O, Troemel ER, Ruvkun G, Bargmann CI 1999. Alternative olfactory neuron fates are specified by the LIM homeobox gene lim-4. Genes Dev 13: 1794-80. PMCID: PMC316880
44. Paradis S, Ailion, M, Toker A, Thomas, JH, and G Ruvkun 1999. A PDK1 homolog is necessary and sufficient to Transduce AGE-1 PI3 kinase signals that regulate diapause in C. elegans. Genes and Development 13: 1438-1452. PMCID: PMC316759
43. Hobert O, Tessmar K and G Ruvkun 1999. The C. elegans lim-6 LIM homeobox gene regulates neurite outgrowth and function of particular GABAergic neurons. Development 126, 1547-1562. PMID: 10068647
42. Hobert O, Moerman DG, Clark KA, Beckerle MC, and G Ruvkun 1999. A conserved LIM protein that affects muscular adherens junction integrity and mechanosensory function in Caenorhabditis elegans. J Cell Biol 144: 45-57. PMCID: PMC2148118
41. Slack F and G Ruvkun 1998. A novel repeat domain that is often associated with RING finger and B-box motifs. Trends Biochem Sci 23:474-5. PMID: 9868369
40. Hobert O and G Ruvkun 1998. A common theme for LIM homeobox gene function across phylogeny? Biol. Bull. 195: 377-80. PMID: 9924780
39. Ogg S and G Ruvkun 1998. The C. elegans PTEN homolog DAF-18 acts in the insulin receptor-like metabolic signaling pathway. Mol Cell 2: 887-93. PMID: 9885576
38. Ruvkun G and O Hobert 1998. The taxonomy of developmental control in Caenorhabditis elegans. Science 282: 2033-41. PMID: 9851920
37. Paradis S and G Ruvkun 1998. Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor. Genes and Development 12: 2488-98. PMCID: PMC317081
36. Hobert O, Liu, Y, D’Alberti, T, and G Ruvkun 1998. Control of neural development and function in a thermoregulatory network by the LIM homeobox gene lin-11. J. Neuroscience 18: 2084-2096. PMID: 9482795
35. Tissenbaum HA and G Ruvkun 1998. An insulin-like signaling pathway affects both longevity and reproduction in C. elegans. Genetics 148: 703-717. PMCID: PMC1459840
34. Ogg S, Paradis, S , Gottlieb, S, Patterson, GI, Lee, L, Tissenbaum, HA, and G Ruvkun 1997. The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature 389: 994-999. PMID: 9353126
33. Kimura KD, Tissenbaum, HA, Liu, Y and G Ruvkun 1997. daf-2, an insulin receptor-like gene that regulates longevity and diapause in C. elegans. Science 277: 942-946. PMID: 9252323
32. Patterson G I, Koweek, A, Wong, A, Liu, Y, and G Ruvkun 1997. The DAF-3 Smad protein antagonizes TGF-beta-related receptor signaling in the C. elegans dauer pathway. Genes and Development 11: 2679-2690. PMCID: PMC316611
31. Hobert O, Mori, I, Yamashita, Y, Honda, H, Ohshima, Y, Liu, Y and G Ruvkun 1997. Regulation of interneuron function in the C. elegans thermoregulatory pathway by the ttx-3 LIM homeobox gene. Neuron 19: 345-357. PMID: 9292724
30. Sluder AE, Lindblom, T and G. Ruvkun 1997. The Caenorhabditis elegans orphan nuclear hormone receptor gene nhr-2 functions in early embryonic development. Developmental Biology 184: 303-319. PMID: 9133437
29. Sze JY, Liu, Y and G Ruvkun 1997. VP16-activation of the C. elegans neural specification transcription factor UNC-86 suppresses mutations in downstream genes and causes defects in neural migration and axon outgrowth. Development 124: 1159-1168. PMID: 9102303
28. Ha I, Wightman, B and G Ruvkun 1996. A bulged lin-4/lin-14 RNA duplex is sufficient for Caenorhabditis elegans lin-14 temporal gradient formation. Genes and Development 10: 3041-3050. PMID: 8957004
27. Barnes TM, Jin, Y, Horvitz, HR, Ruvkun, G, and S Hekimi 1996. The C. elegans behavioral gene unc-24 encodes a novel bipartite protein similar to both erythrocyte band 7.2 (stomatin) and non-specific lipid transfer protein. J. of Neurochemistry 67: 46-57. PMID: 8667025
26. Morris JZ, Tissenbaum, HA and G Ruvkun 1996. A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans. Nature 382: 536-538. PMID: 8700226
25. Baumeister R, Liu, Y, and G Ruvkun 1996. Lineage specific regulators couple cell lineage asymmetry to the transcription of the Caenorhabditis elegans POU gene unc-86 during neurogenesis. Genes and Development 10: 1395-1410. PMID: 8647436
24. Greenstein D, Hird, S Plasterk, R, Andachi, Y, Kohara, Y, Wang, B, Finney, M, and G Ruvkun 1994. Targeted mutations in the C. elegans POU-homeobox gene ceh-18 cause defects in oocyte cell cycle arrest, gonad migration, and epidermal differentiation. Genes and Development 8: 1935-1948. PMID: 7958868
23. Gottlieb S and G Ruvkun 1994. daf-2, daf-16, daf-23: genetically interacting genes controlling dauer formation in C. elegans. Genetics 137: 107-120. PMCID: PMC1205929
22. Wightman B, I Ha, and G Ruvkun 1993. Post-transcriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75: 855-862. PMID: 8252622
21. Bürglin TR and G Ruvkun 1992. New motif in PBX genes. Nature Genet 1: 319‑320. PMID: 1363814
20. Xue DM, Finney, M, Ruvkun, G and M Chalfie 1992. Regulation of the mec-3 gene by the C. elegans homeoproteins Unc-86 and Mec-3. EMBO J. 11: 4969‑4979. PMCID: PMC556975
19. Miller D, Shen, M, Shamu, C, Bürglin, T, Ruvkun, G, Ghee, M Dubois, L Wilson 1992 The C. elegans unc-4 gene encodes a homeodomain protein that determines the pattern of synaptic input to specific motor neurons. Nature 355: 841‑845. PMID: 1347150
18. Bürglin TR, Ruvkun, G, Coulson, A, Hawkins, N, McGhee, J, Schaller, D, Wittmann, C, Müller, F, and Waterston, R 1991. Nematode homeobox cluster. Nature 351: 703. PMID: 1676487
17. Ruvkun G, Wightman, B, Bürglin, TR, and P Arasu. 1991. Dominant gain of function mutations that lead to misregulation of the C. elegans heterochronic gene lin-14, and the evolutionary implications of dominant mutations in pattern‑formation genes. Development (Supplement): 47‑54. PMID: 1742500
16. Arasu P, Wightman, B, G Ruvkun 1991. Temporal regulation of lin-14 by the antagonistic action of two other heterochronic genes, lin-4 and lin-28. Genes and Development 5: 1825‑1833. PMID: 1916265
15. Wightman B, Bürglin, TR, Gatto, J, Arasu, P, G Ruvkun 1991. Negative regulatory sequences in the lin-14 3' untranslated region are necessary to generate a temporal switch during C. elegans development. Genes and Development 5: 1813‑1824. PMID: 1916264
14. Finney M and Ruvkun, G 1990. The unc-86 gene product couples cell lineage and cell identity in Caenorhabditis elegans. Cell 63: 895‑905. PMID: 2257628
13. Ruvkun G, Gilbert, W, Horvitz, HR 1990. Detection of mutations and DNA polymorphisms using whole genome Southern Cross hybridization. Nucleic Acids Research 18: 809‑815. PMCID: PMC330331
12. Bürglin TR, Finney, M, Coulson, A, and Ruvkun, G 1989. C. elegans has scores of homeobox-containing genes. Nature 341: 239‑243. PMID: 2571091
11. Ruvkun G and Giusto, J 1989 The Caenorhabditis elegans heterochronic gene lin-14 encodes a nuclear protein that forms a temporal switch during development. Nature 338: 313‑319. PMID: 2922060
10. Ruvkun G, Ambros, V, Coulson, A, Waterston, R, Sulston, J, and Horvitz, HR 1989. Molecular genetics of the Caenorhabditis elegans heterochronic gene lin-14. Genetics 121: 501‑516. PMCID: PMC1203636
9. Herr W, Sturm, RA, Clerc, RG, Corcoran, LM, Baltimore, D, Sharp, PA, Ingraham, HA, Rosenfeld, MG, Finney, M, Ruvkun, G, and Horvitz, HR 1988 The POU domain: a large conserved region in the mammalian pit-1, oct-1, oct-2, and Caenorhabditis elegans unc-86 gene products. Genes and Development 2: 1513‑1516. PMID: 3215510
8. Finney M, Ruvkun, G, and Horvitz, HR 1988. The Caenorhabditis elegans cell lineage and differentiation gene unc-86 encodes a protein containing a homeodomain and extended similarity to mammalian transcription factors. Cell 55: 757‑769. PMID: 2903797
7. Ruvkun G, Long SR, Meade HM, van den Bos RC, Ausubel FM 1982. ISRml: a Rhizobium meliloti insertion sequence which preferentially transposes into nitrogen fixation (nif) genes. J Mol Appl Genet 1: 405‑418. PMID: 6296251
6. Ruvkun G, Sundaresan V, Ausubel FM 1982. Directed transposon Tn5 mutagenesis and complementation analysis of the Rhizobium meliloti symbiotic nitrogen fixation (nif) genes. Cell 29: 551‑559. PMID: 6288262
5. Ruvkun G, Sundaresan V, Ausubel FM 1982. Specific protection of nucleotides in the lac operator from dimethyl sulfate (DMS) methylation of DNase I nicking by crude bacterial extracts. Gene 18: 245‑255. PMID: 6290328
4. Meade HM, Long SR, Ruvkun G, Brown SE, Ausubel FM 1982. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol 149: 114‑122. PMCID: PMC216598
3. Ruvkun G, Long, SR, Meade, HM, Ausubel, FM 1981. Molecular genetics of symbiotic nitrogen fixation. Cold Spring Harbor Symposium on Quantitative Biology 45: 492‑500.
2. Ruvkun G, Ausubel FM 1981. A general method for site‑directed mutagenesis in prokaryotes. Nature 289: 85‑88.
1. Ruvkun G, Ausubel FM 1980 Interspecies homology of nitrogenase genes. Proc. Natl. Acad. Sci. USA 77: 191‑195. PMCID: PMC348234
Research support
5 R01GM44619-30 NIH Control of C. elegans lineage by heterochronic genes. 05/01/1991 - 01/31/25. 30% effort. Direct Costs $378,999; Total costs $636,718.
2 R01 AG016636-23A1 NIH Genetic and molecular basis of longevity. 20% effort. Project Period: 09/30/1999 to 05/31/2027 Direct Costs $257,119; Total costs $431,960.
5 R01 AG043184-25 NIH Inositol signaling in C. elegans senescence and diapause. 20% effort. 05/01/1996 - 03/31/2023. Direct Costs $281,946 Total Costs $473,668
Friedreich's Ataxia Accelerator. 5% effort. Supports the research on the iron sulfur cluster assembly factor gene frh-1 research. PI: Mootha, Vamsi K. Broad Institute and MGH 09/01/2020 - 06/30/2023. Ruvkun lab portion: $165,528 over 3 years.
Invited Presentations 2018 to 2024
2018 Sanford Burnham Rare Genetic Disease Symposium, San Diego. Genetic and phylogenetic analysis of N to D protein editing of C. elegans N-glycosylated proteins.
2018 RNA bench to bedside, University of Massachusetts. Regulation of viral immunity in C. elegans by eri- and synMuv genes.
2018 National Cancer Institute Distinguished Lecture Series. Regulation of viral immunity in C. elegans by eri- and synMuv genes.
2018 Buck Institute 30 years of Aging Research Symposium. Surveillance of the ribosome and mitochondria in aging and innate immunity.
2018 Breakthrough Discuss, Center for Astrophysics, Harvard. Migration of Life across the Galaxy.
2019 Mars Sample Return Planetary Protection meeting, Jet Propulsion Laboratory, Pasadena Caltech. Migration of Life across the Galaxy.
2019 Breakthrough Discuss,, Berkeley. Migration of Life across the Galaxy.
2019 Canadian Institute for Advanced Research Santa Cruz meeting. Genetic and phylogenetic analysis of N to D protein editing of C. elegans N-glycosylated proteins.
2019 Keystone Meeting on Inflammation and Metabolism, Vancouver BC. Surveillance of the ribosome and mitochondria in aging and innate immunity.
2019 Gordon Research Conference on Aging, Sunday River Maine. Surveillance of the ribosome and mitochondria in aging and innate immunity.
2019 Ketsen Lecture, Andrus Aging Center, USC. Surveillance of the ribosome and mitochondria in aging and innate immunity.
2021 McGill University. Regulation of viral immunity in C. elegans by eri- and synMuv genes.
2021 University of Toronto, Donnelly Genome Centre. Genetic and phylogenetic analysis of N to D protein editing of N-glycosylated proteins in C. elegans.
2021 Cold Spring Harbor Symposium on Quantitative Biology, Biological Timing. Genetic and phylogenetic analysis of N to D protein editing of N-glycosylated proteins in C. elegans.
2021 NO Aging symposium, Oslo Norway (Virtual). Surveillance of the ribosome and mitochondria in aging and innate immunity.
2022 Sydney Brenner Festschrift at Cold Spring Harbor laboratories. Genetic and phylogenetic analysis of N to D protein editing of C. elegans and mammalian viral N-glycosylated proteins.
2022 Don Moerman Symposium at University of British Columbia. Genetic and phylogenetic analysis of N to D protein editing of C. elegans and mammalian viral N-glycosylated proteins.
2022 Vallee Symposium, Portugal. Genetic and phylogenetic analysis of N to D protein editing of C. elegans and mammalian viral N-glycosylated proteins.
2022 Brandeis University Neurobiology and Biology. Migration of Life across the Galaxy.
2022 Vertex Pharmaceuticals. Genetic and phylogenetic analysis of N to D protein editing of C. elegans and mammalian viral N-glycosylated proteins.
2023 Society of Fellows 50th anniversary meeting. Migration of Life across the Galaxy.
2023 George Martin memorial meeting University of Washington. Lifespan regulation and antiviral RNAi in C. elegans insulin signaling mutants.
2023 Duke University Distinguished Lecture series. Lifespan regulation and antiviral RNAi in C. elegans insulin signaling mutants.
Professional Activities
2004 - present Harvard Origins of Life Initiative
2006 - present Broad Institute Associate
2015 - present Chair, Scientific Review Committee, Smith Family Awards Program in Biomedical Research
2015 - present Paul Glenn Medical Foundation Scientific Advisory Board
2018 - present California Life Sciences Company (Calico) Scientific Advisory Board
Past
2003 - 2011 Harvard Microbial Sciences Initiative Organizing committee
2004 - 2007 NIH National Advisory Council on Aging
2012 - 2014 Ellison Medical Foundation Scientific Advisory Board
2015 Vallee Foundation Visiting Professorship
2012 - 2017 NAS Space Studies Board, Committee on Astrobiology and Planetary Science
2017 - 2018 NAS Space Studies Board, Review of Planetary Protection Policy
2015 – 2019 Chair, Genetics Section of the National Academy of Sciences
2018 - 2019 NASA Mars Sample Return Sterilization Working group, Jet Propulsion Laboratory, Caltech
Honorific invited lectures
1998 Nippon Telephone and Telegraph Science Forum Public Lecture, Tokyo
2003 Harvey Lecturer, Rockefeller University
2008 Jean Mitchell Watson Lecture, University of Chicago
2012 Mendel 190th Birthday Symposium, Brno, Czech Republic
2013 60th anniversary of the Double Helix, Cold Spring Harbor
Teaching
Spring 2007 Genetics 206 (with Perrimon, Vidal). 6 lectures and discussion.
Fall and Spring 2007, 2008 Life Sciences 190, Course director, with Schrag, Kolter, Cavanaugh.
2004 to 2012 Co-director, Biology of Aging summer course, Marine Biological Laboratory, Woods Hole
2016 Four lectures Astronomy 305, Topics in Origin of Life Research.
2021 Two lectures, Biophysics 300, Introduction to research